5GQP
Thaumatin Structure at pH 8.0, orthorhombic type1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-06-05 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.964, 53.372, 71.135 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 22.435 - 1.296 |
R-factor | 0.1441 |
Rwork | 0.143 |
R-free | 0.16930 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3vhg |
RMSD bond length | 0.006 |
RMSD bond angle | 1.078 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.320 |
High resolution limit [Å] | 1.296 | 1.300 |
Rmerge | 0.498 | |
Number of reflections | 47852 | |
<I/σ(I)> | 52.33 | 4.67 |
Completeness [%] | 98.1 | 96.4 |
Redundancy | 7.1 | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | PEG3350, 50 mM Tris buffer |