5F2Z
Crystal structure of membrane associated PatA from Mycobacterium smegmatis in complex with palmitate - P21 space group
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-08-10 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97625 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 70.830, 84.480, 98.800 |
Unit cell angles | 90.00, 110.78, 90.00 |
Refinement procedure
Resolution | 92.370 - 2.900 |
R-factor | 0.2407 |
Rwork | 0.239 |
R-free | 0.27370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5f2t |
RMSD bond length | 0.005 |
RMSD bond angle | 0.995 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 92.370 | |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.115 | |
Number of reflections | 23420 | |
<I/σ(I)> | 6.6 | |
Completeness [%] | 96.2 | |
Redundancy | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 100 mM Tris-HCl pH 7.0, 230 mM MgCl2 and 12-16% (w/v) PEG 8,000 |