5E04

Crystal structure of Andes virus nucleoprotein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-05-20
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9798
Spacegroup name	P 21 21 21
Unit cell lengths	52.526, 99.652, 136.388
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.017 - 2.250
R-factor	0.2178
Rwork	0.215
R-free	0.25190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5e06
RMSD bond length	0.008
RMSD bond angle	1.211
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.250
Number of reflections	33842
<I/σ(I)>	23.2
Completeness [%]	90.8
Redundancy	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	100mM HEPES (pH 7.5), 200mM L-proline and 10%(w/v) PEG3350