5CZ3
Crystal Structure of Myxoma Virus M64
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-04-02 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97921 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.782, 70.846, 115.432 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.747 - 2.500 |
R-factor | 0.2079 |
Rwork | 0.207 |
R-free | 0.23200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5cyw |
RMSD bond length | 0.003 |
RMSD bond angle | 0.699 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.540 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.820 | |
Number of reflections | 15289 | |
<I/σ(I)> | 22.6 | 2.5 |
Completeness [%] | 100.0 | 100 |
Redundancy | 9.3 | 8.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 28% w/v PEG MME, 0.1 M Bis-Tris, pH 6.5 |