5COG
Crystal structure of Yeast IRC4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-02-04 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.98 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 146.570, 38.690, 67.100 |
Unit cell angles | 90.00, 108.59, 90.00 |
Refinement procedure
Resolution | 63.599 - 1.613 |
R-factor | 0.1939 |
Rwork | 0.192 |
R-free | 0.23580 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5civ |
RMSD bond length | 0.006 |
RMSD bond angle | 0.972 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHENIX (1.9_1692) |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 63.600 | 1.650 |
High resolution limit [Å] | 1.610 | 1.610 |
Rmerge | 0.071 | 0.576 |
Number of reflections | 45428 | |
<I/σ(I)> | 9.8 | 2.1 |
Completeness [%] | 97.9 | 98.6 |
Redundancy | 4.2 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 90 mM Sodium nitrate, 90 mM Sodium phosphate dibasic, 90 mM Ammonium sulfate, 0.1 M Sodium HEPES, 0.1 M MOPS, pH 7.5, 12% PEG 550 MME, 6% PEG 20,000 |