5BT1
histone chaperone Hif1 playing with histone H2A-H2B dimer
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-02-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97915 |
| Spacegroup name | C 1 2 1 |
| 格子定数 [Å] | 102.673, 48.363, 160.706 |
| 格子定数 [度] | 90.00, 107.05, 90.00 |
精密化法
| 残基 | 43.380 - 2.620 |
| R因子 | 0.2104 |
| Rwork | 0.207 |
| R-free | 0.26180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4nq0 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.008 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.049 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Quality characteristics
| Overall | Inner shell | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 50.000 | 2.670 |
| 分解能 [Å] (高) | 2.620 | 7.110 | 2.620 |
| Rmerge_l_obs | 0.105 | 0.033 | 0.626 |
| Rmeas | 0.123 | 0.038 | 0.734 |
| Rpim | 0.062 | 0.019 | 0.375 |
| Total number of observations | 80873 | ||
| 独立反射数 | 22738 | ||
| <I/σ(I)> | 7.4 | ||
| 完全性 [%] | 98.5 | 95.8 | 98.7 |
| 冗長性 | 3.6 | 3.6 | 3.5 |
| CC(1/2) | 0.998 | 0.683 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 287 | 0.1M Magnesium formate dihydrate, 15% PEG3350 |
