5AJM
H5 (VN1194) Asn186Lys Mutant Haemagglutinin in Complex with Avian Receptor Analogue 3'SLN
Replaces: 4CQTExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Detector | DECTRIS PILATUS |
Spacegroup name | H 3 2 |
Unit cell lengths | 101.580, 101.580, 452.980 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 150.990 - 2.450 |
R-factor | 0.20171 |
Rwork | 0.200 |
R-free | 0.22665 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4bgw |
RMSD bond length | 0.005 |
RMSD bond angle | 1.052 |
Data reduction software | xia2 |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0046) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.790 | 2.580 |
High resolution limit [Å] | 2.450 | 2.450 |
Rmerge | 0.080 | 0.680 |
Number of reflections | 33498 | |
<I/σ(I)> | 13.1 | 2.7 |
Completeness [%] | 99.2 | 98.1 |
Redundancy | 7.8 | 7.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 0.1 M HEPES/MOPS PH 7.0, 0.05 M MGCL2, 28-30% PEG 550 MME, SEEDED WITH CRUSHED WILD-TYPE VN1194 HA CRYSTALS. |