Loading
PDBj
MenuPDBj@FacebookPDBj@TwitterPDBj@YouTubewwPDB FoundationwwPDB
RCSB PDBPDBeBMRBAdv. SearchSearch help

4YKO

Crystal structure of the R111K:Y134F:T54V:R132Q:P39Q:R59Y:A32Y:F3Q mutant of human Cellular Retinoic Acid Binding Protein II with Retinal at 1.58 angstrom resolution

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 21-ID-G
Synchrotron siteAPS
Beamline21-ID-G
Temperature [K]100
Detector technologyCCD
Collection date2013-10-24
DetectorMARMOSAIC 300 mm CCD
Wavelength(s)1.0781
Spacegroup nameP 1
Unit cell lengths36.737, 39.210, 58.402
Unit cell angles86.78, 79.88, 70.83
Refinement procedure
Resolution34.214 - 1.570
R-factor0.1973
Rwork0.196
R-free0.22360
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)2g7b
RMSD bond length0.006
RMSD bond angle1.106
Data reduction softwareHKL-2000
Data scaling softwareHKL-2000
Phasing softwareMOLREP
Refinement softwarePHENIX ((phenix.refine: 1.7.2_869))
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0001.600
High resolution limit [Å]1.5701.570
Rmerge0.0690.288
Number of reflections42116
<I/σ(I)>312.55
Completeness [%]93.370.2
Redundancy3.41.9
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP27720% PEG3350, 0.1M Malonate pH=6.0

225158

數據於2024-09-18公開中

PDB statisticsPDBj update infoContact PDBjnumon