4YFP
CRYSTAL STRUCTURE OF THE R111K:Y134F:T54V:R132Q:P39Y:R59Y MUTANT OF HUMAN CELLULAR RETINOIC ACID BINDING PROTEIN II WITH RETINAL AFTER 20 MINUTES INCUBATION AT 1.95 ANGSTROM RESOLUTION-Kinetic Product
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-10-14 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9787 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 58.935, 58.935, 99.954 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.709 - 1.951 |
R-factor | 0.1875 |
Rwork | 0.185 |
R-free | 0.23360 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2g7b |
RMSD bond length | 0.008 |
RMSD bond angle | 1.185 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine: 1.7.2_869)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.980 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.094 | 0.639 |
Number of reflections | 15215 | |
<I/σ(I)> | 34.6 | 3 |
Completeness [%] | 99.5 | 99.4 |
Redundancy | 10.8 | 10.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 12% PEG3350, 0.1M Malonate pH 6.0 |