4UV9
LSD1(KDM1A)-CoREST in complex with 1-Ethyl-Tranylcypromine
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PIXEL |
| Spacegroup name | I 2 2 2 |
| 格子定数 [Å] | 118.300, 178.560, 234.020 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 53.230 - 3.000 |
| R因子 | 0.22654 |
| Rwork | 0.226 |
| R-free | 0.24585 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v1d |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.005 |
| 結合角の平均二乗偏差(RMSD) [度] | 0.976 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0027) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 53.000 | 3.160 |
| 分解能 [Å] (高) | 3.000 | 3.000 |
| Rmerge_l_obs | 0.140 | 1.060 |
| 独立反射数 | 49581 | |
| <I/σ(I)> | 7.5 | 1.3 |
| 完全性 [%] | 99.5 | 99.8 |
| 冗長性 | 3.4 | 3.6 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | 6.5 | PH 6.5 |
