4RRM
E129A mutant of N-terminal editing domain of threonyl-tRNA synthetase from Aeropyrum pernix with L-Thr3AA
Experimental procedure
実験手法 | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E+ SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2013-03-16 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 41 21 2 |
格子定数 [Å] | 47.091, 47.091, 112.729 |
格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
残基 | 25.000 - 1.550 |
R因子 | 0.19152 |
Rwork | 0.188 |
R-free | 0.25880 |
Structure solution method | MOLECULAR REPLACEMENT |
結合長の平均二乗偏差(RMSD) [Å] | 0.015 |
結合角の平均二乗偏差(RMSD) [度] | 1.744 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0117) |
Quality characteristics
Overall | |
分解能 [Å] (低) | 25.000 |
分解能 [Å] (高) | 1.550 |
独立反射数 | 18120 |
完全性 [%] | 99.3 |
結晶化条件
結晶ID | 方法 | pH | 温度 | 溶液条件 |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 0.1M Tris HCl pH 8.5, 0.2M MgCl2, 30% PEG4000, VAPOR DIFFUSION, HANGING DROP, temperature 277K |