4RRG

Editing domain of threonyl-tRNA synthetase from Methanococcus jannaschii with L-Thr3AA

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2011-08-16
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
格子定数 [Å]	36.569, 64.885, 97.752
格子定数 [度]	90.00, 91.07, 90.00

精密化法

残基	24.550 - 1.930
R因子	0.19781
Rwork	0.196
R-free	0.23220
Structure solution method	MOLECULAR REPLACEMENT
結合長の平均二乗偏差(RMSD) [Å]	0.009
結合角の平均二乗偏差(RMSD) [度]	1.185
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0109)

Quality characteristics

	Overall
分解能 [Å] (低)	25.000
分解能 [Å] (高)	1.930
独立反射数	32739
完全性 [%]	99.3

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	0.1M Tris HCl, 0.2M MgCl2, 25% PEG3350, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K