4RRF

Editing domain of threonyl-tRNA synthetase from Methanococcus jannaschii with L-Ser3AA

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2011-05-06
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
格子定数 [Å]	159.595, 52.813, 98.315
格子定数 [度]	90.00, 104.04, 90.00

精密化法

残基	25.000 - 1.700
R因子	0.197
Rwork	0.194
R-free	0.23900
Structure solution method	MOLECULAR REPLACEMENT
結合長の平均二乗偏差(RMSD) [Å]	0.015
結合角の平均二乗偏差(RMSD) [度]	1.782
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.6.0117)

Quality characteristics

	Overall
分解能 [Å] (低)	25.000
分解能 [Å] (高)	1.700
独立反射数	75775
完全性 [%]	91.0

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	0.1M Tris HCl, 0.2M MgCl2, 25% PEG3350, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K