4QDJ
Crystal structure of magnesium protoporphyrin IX methyltransferase (ChlM) from Synechocystis PCC 6803 with bound SAM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-04-10 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9793 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.319, 61.026, 68.892 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.514 - 1.600 |
R-factor | 0.1434 |
Rwork | 0.141 |
R-free | 0.17970 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.840 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (1.8.1_1168) |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.660 |
High resolution limit [Å] | 1.600 | 1.600 |
Number of reflections | 27515 | |
Completeness [%] | 97.9 | 84.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 277 | 0.1M MES pH 6.7, 10% PEG 3350, 200mM NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 277 | 0.1M MES pH 6.7, 10% PEG 3350, 200mM NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 277K |