4QB0

The crystal structure of the C-terminal domain of Ebola (Zaire) nucleoprotein

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2013-10-31
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
格子定数 [Å]	36.599, 49.169, 53.806
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	29.359 - 1.750
R因子	0.185
Rwork	0.183
R-free	0.22490
Structure solution method	MOLECULAR REPLACEMENT
結合長の平均二乗偏差(RMSD) [Å]	0.013
結合角の平均二乗偏差(RMSD) [度]	1.315
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	35.000	1.780
分解能 [Å] (高)	1.750	1.750
独立反射数	9991
<I/σ(I)>	31.6	1.74
完全性 [%]	97.7	82.2
冗長性	6.3	3.2

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, SITTING DROP		293	19.3% PEG3350, 0.05M Magnesium Formate, VAPOR DIFFUSION, SITTING DROP, temperature 293K