4NJ5

Crystal structure of SUVH9

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-06
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.2823
Spacegroup name	P 21 21 21
格子定数 [Å]	63.679, 84.197, 99.359
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	49.679 - 2.400
R因子	0.1886
Rwork	0.187
R-free	0.22260
Structure solution method	SAD
結合長の平均二乗偏差(RMSD) [Å]	0.007
結合角の平均二乗偏差(RMSD) [度]	0.961
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	50.000	2.490
分解能 [Å] (高)	2.400	2.400
Rmerge_l_obs	0.100	0.698
独立反射数	21408
<I/σ(I)>	35	3.5
完全性 [%]	98.3	100
冗長性	14	14.4

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.2 M potassium thiocyanate, 0.1 M Bis-Tris propane, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K