4LD9
Crystal structure of the N-terminally acetylated BAH domain of Sir3 bound to the nucleosome core particle
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2013-05-25 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.96863 |
| Spacegroup name | P 61 |
| Unit cell lengths | 105.513, 105.513, 488.225 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.997 - 3.306 |
| R-factor | 0.2405 |
| Rwork | 0.238 |
| R-free | 0.29460 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.597 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASES |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 3.200 |
| Number of reflections | 44335 |
| Completeness [%] | 90.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 293.15 | 50 mM MES pH 6.5, 12% PEG 400, 12 mM MnCl2, 100 mM NaCl, 10 mM EDTA, VAPOR DIFFUSION, temperature 293.15K |
