4LAO
Crystal structure of Cordyceps militaris IDCase H195A mutant (Zn)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-19 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 144.365, 57.510, 105.563 |
| Unit cell angles | 90.00, 105.31, 90.00 |
Refinement procedure
| Resolution | 45.330 - 2.000 |
| R-factor | 0.1704 |
| Rwork | 0.169 |
| R-free | 0.20520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hk5 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.978 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
| Rmerge | 0.062 | 0.031 | 0.355 |
| Number of reflections | 55498 | ||
| <I/σ(I)> | 11.2 | ||
| Completeness [%] | 97.9 | 97.3 | 97.1 |
| Redundancy | 4.2 | 4 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | sitting drop | 5.6 | 289 | 30% polyethylene glycol 4000, 0.2M NH4Ac, 2mM ZnCl2, 0.1M sodium citrate, pH 5.6, sitting drop, temperature 289K |
