4JNT
Crystal structure of the ectodomain of Bovine viral diarrhea virus 1 E2 envelope protein
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 195 |
| Detector technology | CCD |
| Collection date | 2011-04-06 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | C 1 2 1 |
| 格子定数 [Å] | 155.632, 67.885, 139.406 |
| 格子定数 [度] | 90.00, 121.89, 90.00 |
精密化法
| 残基 | 48.570 - 4.090 |
| R因子 | 0.323 |
| Rwork | 0.321 |
| R-free | 0.37600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ild |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.012 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.894 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 4.340 |
| 分解能 [Å] (高) | 4.090 | 4.090 |
| Rmerge_l_obs | 1.002 | |
| 独立反射数 | 18935 | |
| <I/σ(I)> | 4.78 | 1.34 |
| 完全性 [%] | 98.1 | 98.1 |
| 冗長性 | 2.12 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | 5.5 | 294 | 10% polyethylene glycol 3350, 0.1 M Bis-tris pH 5.5, 0.05 M cesium chloride, 0.04 M calcium acetate, 10% glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
