4JA9

Rat PP5 apo

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-10-21
Detector	PSI PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 41 21 2
格子定数 [Å]	50.090, 50.090, 379.190
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	15.000 - 2.300
R因子	0.23931
Rwork	0.238
R-free	0.27172
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ja7
結合長の平均二乗偏差(RMSD) [Å]	0.005
結合角の平均二乗偏差(RMSD) [度]	0.991
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.7.0029)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	40.000	2.400
分解能 [Å] (高)	2.300	2.300
Rmerge_l_obs	0.041	0.537
独立反射数	22932
<I/σ(I)>	22.3	4.4
完全性 [%]	99.9	99.9
冗長性	6.1

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	7.8	293	0.2 M Mg(NO3)2, 20% PEG 3350, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K