4IK9
High resolution structure of GCaMP3 dimer form 2 at pH 7.5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-04-05 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9794 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 125.617, 46.749, 68.753 |
Unit cell angles | 90.00, 100.06, 90.00 |
Refinement procedure
Resolution | 26.000 - 1.800 |
R-factor | 0.16128 |
Rwork | 0.159 |
R-free | 0.19497 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.021 |
RMSD bond angle | 1.883 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.000 | 1.420 |
High resolution limit [Å] | 1.400 | 1.400 |
Number of reflections | 76958 | |
Completeness [%] | 98.9 | 95.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | HEPES pH 7.5, (NH4)2SO4, 21% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 289K |