4HYD

Structure of a presenilin family intramembrane aspartate protease in C2221 space group

Experimental procedure

実験手法	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2011-04-12
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.07171, 1.05384, 1.07206
Spacegroup name	C 2 2 21
格子定数 [Å]	114.077, 170.025, 181.397
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	31.577 - 3.800
R因子	0.3073
Rwork	0.307
R-free	0.30810
Structure solution method	MAD
結合長の平均二乗偏差(RMSD) [Å]	0.010
結合角の平均二乗偏差(RMSD) [度]	1.663
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Quality characteristics

	Overall
分解能 [Å] (低)	50.000
分解能 [Å] (高)	3.800
独立反射数	15330

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	3.6	291	0.1M Glycine, 0.2M (NH4)2SO4, 20%(w/v) PEG500MME, 6%(w/v) Glycerol, 0.04%(w/v) Anapoe-C12E8, pH 3.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K