4FT2
crystal structure of Zea mays ZMET2 in complex H3(1-15)K9me2 peptide and SAH
Experimental procedure
実験手法 | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-07-14 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9792 |
Spacegroup name | P 1 |
格子定数 [Å] | 64.861, 88.858, 113.501 |
格子定数 [度] | 93.15, 95.72, 110.70 |
精密化法
残基 | 40.099 - 3.200 |
R因子 | 0.2373 |
Rwork | 0.235 |
R-free | 0.27730 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4fsx |
結合長の平均二乗偏差(RMSD) [Å] | 0.009 |
結合角の平均二乗偏差(RMSD) [度] | 1.575 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Quality characteristics
Overall | Outer shell | |
分解能 [Å] (低) | 50.000 | 3.310 |
分解能 [Å] (高) | 3.200 | 3.200 |
Rmerge_l_obs | 0.118 | 0.503 |
独立反射数 | 38805 | |
<I/σ(I)> | 12.6 | 2.2 |
完全性 [%] | 99.0 | 98.8 |
冗長性 | 3.4 | 3.4 |
結晶化条件
結晶ID | 方法 | pH | 温度 | 溶液条件 |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 0.2 M calcium acetate, 0.1 M imidazole pH 8.0, and 10% PEG8000, VAPOR DIFFUSION, HANGING DROP, temperature 277K |