4FSX
crystal structure of Se-substituted Zea mays ZMET2 in complex with SAH
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 |
| 格子定数 [Å] | 64.857, 88.952, 113.493 |
| 格子定数 [度] | 93.47, 95.53, 110.41 |
精密化法
| 残基 | 40.250 - 3.200 |
| R因子 | 0.241 |
| Rwork | 0.240 |
| R-free | 0.26300 |
| Structure solution method | SAD |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.013 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.819 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 3.310 |
| 分解能 [Å] (高) | 3.200 | 3.200 |
| Rmerge_l_obs | 0.175 | 0.595 |
| 独立反射数 | 38702 | |
| <I/σ(I)> | 7.2 | 1.6 |
| 完全性 [%] | 99.2 | 98.8 |
| 冗長性 | 3.7 | 3.6 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 0.2 M calcium acetate, 0.1 M imidazole pH 8.0, and 10% PEG8000, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
