4ET9
Hen egg-white lysozyme solved from 5 fs free-electron laser pulse data
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | FREE ELECTRON LASER |
Source details | SLAC LCLS BEAMLINE CXI |
Synchrotron site | SLAC LCLS |
Beamline | CXI |
Temperature [K] | 293 |
Detector technology | PIXEL |
Collection date | 2011-02-01 |
Wavelength(s) | 1.32 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 79.000, 79.000, 38.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.300 - 1.900 |
R-factor | 0.1909 |
Rwork | 0.189 |
R-free | 0.22650 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.006 |
RMSD bond angle | 1.025 |
Data reduction software | CrystFEL |
Data scaling software | CrystFEL |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.7.2_869)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.300 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 9743 | |
Completeness [%] | 98.2 | 91.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH | 293 | After crystallization, the solution used for growing the crystals (20 % NaCl, 6 % PEG 6000, 1 M Na acetate pH 3.0) was exchanged for storage solution (10 % NaCl, 1.0 M Na acetate pH 3.4), BATCH, temperature 293K |