4C6C
Crystal structure of the dihydroorotase domain of human CAD in apo- form obtained recombinantly from HEK293 cells.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-12-12 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 82.110, 158.800, 60.760 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.055 - 1.451 |
R-factor | 0.1238 |
Rwork | 0.122 |
R-free | 0.14780 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4by3 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.336 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.250 | 1.490 |
High resolution limit [Å] | 1.450 | 1.450 |
Rmerge | 0.080 | 0.580 |
Number of reflections | 69725 | |
<I/σ(I)> | 17.2 | 3.2 |
Completeness [%] | 99.0 | 90.6 |
Redundancy | 9.5 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | pH 7 |