402D
5'-R(*CP*GP*CP*CP*AP*GP*CP*G)-3'
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-01-01 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | H 3 2 |
| 格子定数 [Å] | 42.737, 42.737, 123.900 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 8.000 - 2.300 |
| R因子 | 0.207 |
| Rwork | 0.207 |
| R-free | 0.26300 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.035 * |
| 結合角の平均二乗偏差(RMSD) [度] | 0.867 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 30.000 | |
| 分解能 [Å] (高) | 2.300 | 2.300 * |
| Rmerge_l_obs | 0.044 | 0.340 * |
| Total number of observations | 25094 * | |
| 独立反射数 | 2101 | |
| 完全性 [%] | 96.8 | 80.2 * |
| 冗長性 | 11.9 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | drop solution was mixed with an equal volume of reservoir solution * |
結晶化試薬
| ID | 結晶ID | 濃度 | 試薬名 | 濃度 (単位) | 詳細 |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | NACL | ||
| 3 | 1 | 1 | MGCL2 | ||
| 4 | 1 | 1 | SPERMINE TETRAHYDROCHLORIDE | ||
| 5 | 1 | 1 | PRECIPITATING SOLUTION | ||
| 6 | 1 | 2 | MPD | ||
| 7 | 1 | 2 | NA CACODYLATE |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | drop | RNA | 2 (mM) | |
| 2 | 1 | reservoir | 20 (mM) | ||
| 3 | 1 | reservoir | 5 (mM) | ||
| 4 | 1 | reservoir | spermine tetrahydrochloride | 2 (mM) | |
| 5 | 1 | reservoir | MPD | 35 (%) | |
| 6 | 1 | reservoir | sodium cacodylate | 100 (mM) | precipitant |
