402D
5'-R(*CP*GP*CP*CP*AP*GP*CP*G)-3'
Experimental procedure
実験手法 | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1998-01-01 |
Detector | RIGAKU RAXIS IIC |
Spacegroup name | H 3 2 |
格子定数 [Å] | 42.737, 42.737, 123.900 |
格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
残基 | 8.000 - 2.300 |
R因子 | 0.207 |
Rwork | 0.207 |
R-free | 0.26300 |
結合長の平均二乗偏差(RMSD) [Å] | 0.035 * |
結合角の平均二乗偏差(RMSD) [度] | 0.867 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | CNS |
Quality characteristics
Overall | Outer shell | |
分解能 [Å] (低) | 30.000 | |
分解能 [Å] (高) | 2.300 | 2.300 * |
Rmerge_l_obs | 0.044 | 0.340 * |
Total number of observations | 25094 * | |
独立反射数 | 2101 | |
完全性 [%] | 96.8 | 80.2 * |
冗長性 | 11.9 |
結晶化条件
結晶ID | 方法 | pH | 温度 | 溶液条件 |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | drop solution was mixed with an equal volume of reservoir solution * |
結晶化試薬
ID | 結晶ID | 濃度 | 試薬名 | 濃度 (単位) | 詳細 |
1 | 1 | 1 | WATER | ||
2 | 1 | 1 | NACL | ||
3 | 1 | 1 | MGCL2 | ||
4 | 1 | 1 | SPERMINE TETRAHYDROCHLORIDE | ||
5 | 1 | 1 | PRECIPITATING SOLUTION | ||
6 | 1 | 2 | MPD | ||
7 | 1 | 2 | NA CACODYLATE |
文献の結晶化試薬
ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
1 | 1 | drop | RNA | 2 (mM) | |
2 | 1 | reservoir | 20 (mM) | ||
3 | 1 | reservoir | 5 (mM) | ||
4 | 1 | reservoir | spermine tetrahydrochloride | 2 (mM) | |
5 | 1 | reservoir | MPD | 35 (%) | |
6 | 1 | reservoir | sodium cacodylate | 100 (mM) | precipitant |