3ZRY
Rotor architecture in the F(1)-c(10)-ring complex of the yeast F-ATP synthase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-02-07 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 107.690, 174.910, 164.260 |
Unit cell angles | 90.00, 93.92, 90.00 |
Refinement procedure
Resolution | 54.625 - 6.500 |
R-factor | 0.3178 |
Rwork | 0.317 |
R-free | 0.33870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2xok |
RMSD bond length | 0.003 |
RMSD bond angle | 0.944 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.620 | 6.850 |
High resolution limit [Å] | 6.500 | 6.500 |
Rmerge | 0.120 | 0.920 |
Number of reflections | 11687 | |
<I/σ(I)> | 8.9 | 1.8 |
Completeness [%] | 96.6 | 97.2 |
Redundancy | 3.5 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 10% PEG 4000, 100 MM SODIUM CHLORIDE, 100 MM HEPES PH 6.5 MIXED 1:1 WITH PROTEIN SOLUTION (10 MG/ML) CONTAINING 0.64 MM DDM, 25 MM TRIS PH 8.0, 100 MM SODIUM CHLORIDE, 25 MM TREHALOSE, 0.5 MM EDTA, 3 MM SODIUM AZIDE, 2 MM MAGNESIUM CHLORIDE, 0.04 MM ADP, 1 MM AMP-PNP, 0.1 MM DCCD, 2.5 MM DTT, 0.5 MM PMSF. |