3ZE5
Crystal structure of the integral membrane diacylglycerol kinase - delta4
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-12-11 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 31 2 1 |
| 格子定数 [Å] | 72.740, 72.740, 198.990 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 45.678 - 3.101 |
| R因子 | 0.2335 |
| Rwork | 0.232 |
| R-free | 0.25810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ze3 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.003 |
| 結合角の平均二乗偏差(RMSD) [度] | 0.540 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 60.050 | 3.180 |
| 分解能 [Å] (高) | 3.100 | 3.100 |
| Rmerge_l_obs | 0.060 | 0.600 |
| 独立反射数 | 11008 | |
| <I/σ(I)> | 9.9 | 2.1 |
| 完全性 [%] | 95.0 | 90.9 |
| 冗長性 | 2.9 | 2.8 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | LIPIDIC CUBIC PHASE | 5.6 | 277 | 3-5 %(V/V) 2-METHYL-2, 4-PENTANEDIOL, 0.1 M SODIUM CHLORIDE, 0.1 M LITHIUM NITRATE, 0.1 M SODIUM CITRATE/HCL PH 5.6. CRYSTALLIZED USING THE IN MESO (LIPIDIC CUBIC PHASE) METHOD AT 4 DEGREE CELSIUS WITH THE 7.8 MONOACYLGLYCEROL (7.8 MAG) AS THE HOSTING LIPID. |
