3WOY

Crystal structure of CLASP2 TOG domain (TOG2)

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	100
Detector technology	CCD
Collection date	2009-02-18
Detector	ADSC QUANTUM 4r
Wavelength(s)	0.97901, 0.97931, 0.96408
Spacegroup name	P 32 2 1
Unit cell lengths	58.812, 58.812, 157.073
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.730 - 2.100
R-factor	0.183
Rwork	0.183
R-free	0.23700
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	1.100
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.3)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.010
High resolution limit [Å]	2.000	4.180	2.000
Number of reflections	44169
Completeness [%]	99.9	99.1	99.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	20mM Tris, 3%(w/v) PEG10K , pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	20mM Tris, 3%(w/v) PEG10K , pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K