3VJ7

Crystal structure of the carboxy-terminal ribonuclease domain of Colicin E5 R33Q mutant

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	MACSCIENCE
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1999-11-28
Detector	MAC Science DIP-2030
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
格子定数 [Å]	29.688, 49.450, 64.619
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	39.270 - 2.300
R因子	0.23888
Rwork	0.238
R-free	0.25730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2djh
結合長の平均二乗偏差(RMSD) [Å]	0.013
結合角の平均二乗偏差(RMSD) [度]	1.833
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	50.000	2.380
分解能 [Å] (高)	2.300	2.300
独立反射数	4574
完全性 [%]	99.8	98.8

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	8	293	2M ammonium sulfate, 20mM TrisCl, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 293K