3UQ3

TPR2AB-domain:pHSP90-complex of yeast Sti1

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR
Temperature [K]	100
Detector technology	CCD
Collection date	2011-01-14
Detector	Bruker Platinum 135
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
格子定数 [Å]	43.191, 62.897, 66.842
格子定数 [度]	90.00, 95.80, 90.00

精密化法

残基	10.000 - 2.600
R因子	0.22027
Rwork	0.217
R-free	0.27854
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1elr
結合長の平均二乗偏差(RMSD) [Å]	0.006
結合角の平均二乗偏差(RMSD) [度]	0.979
Data reduction software	PROTEUM2
Data scaling software	PROTEUM2
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	40.000	2.700
分解能 [Å] (高)	2.600	2.600
Rmerge_l_obs	0.082	0.352
独立反射数	17924
<I/σ(I)>	15.26	3.2
完全性 [%]	99.0	97.2

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	8.8	293	0.2 M TMAO, 0.1 M Tris, 20% PEG 2000 MME, pH 8.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K