3RAA
Structural studies of AAV8 capsid transitions associated with endosomal trafficking
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 113 |
Detector technology | CCD |
Collection date | 2008-06-13 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.990 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 256.400, 256.400, 447.430 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.840 - 3.200 |
Rwork | 0.294 |
R-free | 0.29730 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 3.310 |
High resolution limit [Å] | 3.200 | 3.200 |
Number of reflections | 126615 | |
Completeness [%] | 89.0 | 88.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 20 mM Tris pH 7.5, 4% PEG 4000, 1 M NaCl. Crystals were pre-soaked at pH 4 for 24 hours and transferred back to pH7.5 before data collection, VAPOR DIFFUSION, HANGING DROP, temperature 293K |