3RA9
Structural studies of AAV8 capsid transitions associated with endosomal trafficking
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-07-06 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 63 2 2 |
| 格子定数 [Å] | 257.940, 257.940, 448.690 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 40.000 - 2.700 |
| R因子 | 0.244 |
| Rwork | 0.243 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.010 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | CNS |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 40.000 | 2.800 |
| 分解能 [Å] (高) | 2.700 | 2.700 |
| 独立反射数 | 175411 | |
| 完全性 [%] | 73.5 | 29.4 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 20 mM Tris pH 7.5, 4% PEG 8000, 1 M NaCl, Crystals were pre-soaked in pH6 cryosolvent for 24 hours, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
