3Q26
Cyrstal structure of human alpha-synuclein (10-42) fused to maltose binding protein (MBP)
Experimental procedure
実験手法 | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-10-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97949 |
Spacegroup name | P 21 21 21 |
格子定数 [Å] | 48.740, 57.490, 127.230 |
格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
残基 | 63.615 - 1.540 |
R因子 | 0.142 |
Rwork | 0.140 |
R-free | 0.18310 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1anf |
結合長の平均二乗偏差(RMSD) [Å] | 0.009 |
結合角の平均二乗偏差(RMSD) [度] | 1.180 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.1.4) |
Refinement software | PHENIX (1.6.4_486) |
Quality characteristics
Overall | Inner shell | Outer shell | |
分解能 [Å] (低) | 63.615 | 1.580 | |
分解能 [Å] (高) | 1.540 | 6.890 | 1.540 |
Rmerge_l_obs | 0.077 | 0.039 | 0.561 |
独立反射数 | 53765 | 697 | 3911 |
<I/σ(I)> | 17.72 | 36.8 | 3.6 |
完全性 [%] | 100.0 | 100 | 99.9 |
結晶化条件
結晶ID | 方法 | pH | 温度 | 溶液条件 |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 290 | 0.2M tri-lithium citrate, 2.2 M ammonium sulfate, pH 8.0, vapor diffusion, hanging drop, temperature 290K |