3PFT
Crystal Structure of Untagged C54A Mutant Flavin Reductase (DszD) in Complex with FMN From Mycobacterium goodii
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-10-24 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1 |
Spacegroup name | I 2 3 |
Unit cell lengths | 131.174, 131.174, 131.174 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.058 - 1.601 |
R-factor | 0.1743 |
Rwork | 0.173 |
R-free | 0.19600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.194 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.650 |
High resolution limit [Å] | 1.600 | 3.560 | 1.600 |
Rmerge | 0.059 | 0.028 | 0.593 |
Number of reflections | 49333 | ||
<I/σ(I)> | 10.7 | ||
Completeness [%] | 99.9 | 99.4 | 100 |
Redundancy | 15.2 | 15.2 | 14.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1M Bis-Tris, 34% PEG 3350, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |