3PFT
Crystal Structure of Untagged C54A Mutant Flavin Reductase (DszD) in Complex with FMN From Mycobacterium goodii
Experimental procedure
実験手法 | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-10-24 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1 |
Spacegroup name | I 2 3 |
格子定数 [Å] | 131.174, 131.174, 131.174 |
格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
残基 | 35.058 - 1.601 |
R因子 | 0.1743 |
Rwork | 0.173 |
R-free | 0.19600 |
Structure solution method | MOLECULAR REPLACEMENT |
結合長の平均二乗偏差(RMSD) [Å] | 0.009 |
結合角の平均二乗偏差(RMSD) [度] | 1.194 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | PHENIX |
Quality characteristics
Overall | Inner shell | Outer shell | |
分解能 [Å] (低) | 50.000 | 50.000 | 1.650 |
分解能 [Å] (高) | 1.600 | 3.560 | 1.600 |
Rmerge_l_obs | 0.059 | 0.028 | 0.593 |
独立反射数 | 49333 | ||
<I/σ(I)> | 10.7 | ||
完全性 [%] | 99.9 | 99.4 | 100 |
冗長性 | 15.2 | 15.2 | 14.1 |
結晶化条件
結晶ID | 方法 | pH | 温度 | 溶液条件 |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1M Bis-Tris, 34% PEG 3350, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |