3O17

Crystal Structure of JNK1-alpha1 isoform

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2004-10-17
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0
Spacegroup name	P 32 2 1
格子定数 [Å]	155.850, 155.850, 124.492
格子定数 [度]	90.00, 90.00, 120.00

精密化法

残基	19.940 - 3.000
R因子	0.263
Rwork	0.263
R-free	0.30800
Structure solution method	MOLECULAR REPLACEMENT
結合長の平均二乗偏差(RMSD) [Å]	0.011
結合角の平均二乗偏差(RMSD) [度]	1.600
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS (1.2)

Quality characteristics

	Overall
分解能 [Å] (低)	50.000
分解能 [Å] (高)	3.000
独立反射数	31697
完全性 [%]	90.1

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	6.2	277	Hanging drop : 2ul of 10 mg/ml protein plus 2 ul of well solution Well solution : 3.0 M ammonium sulfate, 10 % glycerol, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 277K