3NEC
Crystal Structure of Toxoplasma gondii Profilin
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 |
| 格子定数 [Å] | 49.528, 53.335, 68.617 |
| 格子定数 [度] | 74.70, 73.82, 68.98 |
精密化法
| 残基 | 26.300 - 1.700 |
| Rwork | 0.159 |
| R-free | 0.19618 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2JKF without residues 57-77 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.011 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.168 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0077) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 27.000 | 1.760 |
| 分解能 [Å] (高) | 1.700 | 1.700 |
| Rmerge_l_obs | 0.073 | 0.410 |
| 独立反射数 | 61203 | |
| <I/σ(I)> | 17.18 | 3 |
| 完全性 [%] | 94.0 | 69.9 |
| 冗長性 | 3.8 | 3.5 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 289 | 2.4 M ammonium sulfate, 0.1 M sodium citrate, 0.2 M potassium/sodium tartrate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 289.0K |
