3N24
The crystal structure of a two-component sensor domain (3rd form) from Pseudomonas aeruginosa PA01
Experimental procedure
実験手法 | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-16 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97931 |
Spacegroup name | P 1 |
格子定数 [Å] | 48.003, 61.093, 71.600 |
格子定数 [度] | 85.60, 70.47, 89.50 |
精密化法
残基 | 36.630 - 2.056 |
R因子 | 0.2064 |
Rwork | 0.203 |
R-free | 0.28160 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3kkb |
結合長の平均二乗偏差(RMSD) [Å] | 0.007 |
結合角の平均二乗偏差(RMSD) [度] | 1.022 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Quality characteristics
Overall | Outer shell | |
分解能 [Å] (低) | 37.000 | 2.090 |
分解能 [Å] (高) | 2.050 | 2.050 |
Rmerge_l_obs | 0.081 | 0.549 |
独立反射数 | 46030 | |
<I/σ(I)> | 14.4 | 1.54 |
完全性 [%] | 97.2 | 90 |
冗長性 | 2.2 | 2.2 |
結晶化条件
結晶ID | 方法 | pH | 温度 | 溶液条件 |
1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 293 | 0.2M sodium, 0.1M phosphate citrate, 20% PEG8000, pH 4.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |