3LKX
Human nac dimerization domain
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BSRF BEAMLINE 3W1A |
| Synchrotron site | BSRF |
| Beamline | 3W1A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-16 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 61 2 2 |
| 格子定数 [Å] | 59.755, 59.755, 176.823 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 19.560 - 2.500 |
| R因子 | 0.218 |
| Rwork | 0.215 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tr8 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.009 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.374 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Quality characteristics
| Overall | Inner shell | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 50.000 | 2.540 |
| 分解能 [Å] (高) | 2.500 | 6.780 | 2.500 |
| Rmerge_l_obs | 0.067 | 0.041 | 0.443 |
| 独立反射数 | 7011 | ||
| <I/σ(I)> | 9.1 | ||
| 完全性 [%] | 98.8 | 98.9 | 86.4 |
| 冗長性 | 17.1 | 16 | 5.3 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 285 | PEG 6000, Sodium citrate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 285K |
