3L34
The crystal structure of a two-component sensor domain (2nd form) from Pseudomonas aeruginosa PA01
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 1 21 1 |
| 格子定数 [Å] | 61.506, 103.081, 69.465 |
| 格子定数 [度] | 90.00, 90.01, 90.00 |
精密化法
| 残基 | 41.392 - 1.700 |
| R因子 | 0.1843 |
| Rwork | 0.182 |
| R-free | 0.22510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kkb |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.006 |
| 結合角の平均二乗偏差(RMSD) [度] | 0.904 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP (CCP4) |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 42.000 | 1.730 |
| 分解能 [Å] (高) | 1.700 | 1.700 |
| Rmerge_l_obs | 0.068 | 0.735 |
| 独立反射数 | 93984 | |
| <I/σ(I)> | 26.1 | 1.5 |
| 完全性 [%] | 99.5 | 94.7 |
| 冗長性 | 4.5 | 3.8 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 289 | 0.1M Ammonium acetate 0.1M Sodium acettae 30% PEG4000, 15% glycerol, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
