3KUU
Structure of the PurE Phosphoribosylaminoimidazole Carboxylase Catalytic Subunit from Yersinia pestis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2009-11-13 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.127 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 114.040, 114.040, 231.740 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.300 - 1.410 |
R-factor | 0.151 |
Rwork | 0.150 |
R-free | 0.16700 |
Structure solution method | SAD |
RMSD bond length | 0.018 |
RMSD bond angle | 1.631 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.450 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.104 | 0.518 |
Number of reflections | 145751 | |
<I/σ(I)> | 14.2 | 3 |
Completeness [%] | 99.1 | 93.5 |
Redundancy | 11.8 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 1.6M ammonium sulphate, 4% maltose, 0.1M HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 1.6M ammonium sulphate, 4% maltose, 0.1M HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 1.6M ammonium sulphate, 4% maltose, 0.1M HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |