3KPH

Crystal structure of Mycoplasma arthritidis-derived mitogen

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2004-09-10
Detector	ADSC QUANTUM 4
Wavelength(s)	1.2757
Spacegroup name	P 41 3 2
格子定数 [Å]	181.865, 181.865, 181.865
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	50.000 - 2.800
R因子	0.253
Rwork	0.253
R-free	0.29900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1r5i
結合長の平均二乗偏差(RMSD) [Å]	0.008
結合角の平均二乗偏差(RMSD) [度]	1.400
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	PHASER
Refinement software	CNS (1.0)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	50.000	2.900
分解能 [Å] (高)	2.800	2.800
Rmerge_l_obs	0.117	0.692
独立反射数	24867
<I/σ(I)>	9.6	2.7
完全性 [%]	95.9	96.8
冗長性	6.93	5.4

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION	6.2	293	13-15% PEG 3350, 0.2 M NaCl, 5% Ethylene glycol, 5% Glycerol, 0.1 M Potassium/sodium phosphate pH 6.2, VAPOR DIFFUSION, temperature 293K