3JZY
Crystal structure of human Intersectin 2 C2 domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-08-05 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97711 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 54.125, 54.125, 196.709 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.560 |
R-factor | 0.223 |
Rwork | 0.222 |
R-free | 0.23500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ep6 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.607 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.000 | 40.000 | 1.590 |
High resolution limit [Å] | 1.560 | 4.230 | 1.560 |
Rmerge | 0.079 | 0.045 | 0.890 |
Number of reflections | 21447 | ||
<I/σ(I)> | 13.4 | ||
Completeness [%] | 99.9 | 97.9 | 100 |
Redundancy | 17.7 | 15.7 | 15.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 291 | 20% PEG3350, 0.2M calcium chloride. calcium chloride was also added to the protein stock solution to a final concentration of 0.002M. Crystals were detected only after more than 100 days of incubation., vapor diffusion, temperature 291K |