3JZI

Crystal structure of biotin carboxylase from E. Coli in complex with benzimidazole series

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2006-01-01
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
格子定数 [Å]	84.758, 107.816, 121.941
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	19.970 - 2.310
Rwork	0.187
R-free	0.22310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bnc
Data reduction software	DENZO
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	BUSTER (2.9.2)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	20.000	2.380
分解能 [Å] (高)	2.300	2.300
独立反射数	47651
完全性 [%]	97.5	98.5

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, SITTING DROP	8	295	Co-crystallization. 8-10% PEG6000, 100mM Tris-HCl., VAPOR DIFFUSION, SITTING DROP, temperature 295K