3JU8

Crystal Structure of Succinylglutamic Semialdehyde Dehydrogenase from Pseudomonas aeruginosa.

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2009-08-19
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9788
Spacegroup name	C 1 2 1
格子定数 [Å]	137.117, 48.033, 187.130
格子定数 [度]	90.00, 107.43, 90.00

精密化法

残基	32.775 - 1.820
R因子	0.16
Rwork	0.158
R-free	0.19600
Structure solution method	SAD
結合長の平均二乗偏差(RMSD) [Å]	0.013
結合角の平均二乗偏差(RMSD) [度]	1.464
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	HKL-3000
Refinement software	PHENIX ((phenix.refine: 1.4_138))

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	32.780	1.850
分解能 [Å] (高)	1.820	1.820
独立反射数	104456
<I/σ(I)>	12.5	2.9
完全性 [%]	99.3	91.9
冗長性	4	3.2

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, SITTING DROP	6.5	289	1.6 M Magnesium sulfate 0.1 M MES pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K