3JRS
Crystal structure of (+)-ABA-bound PYL1
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 41 21 2 |
| 格子定数 [Å] | 97.730, 97.730, 135.270 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 20.000 - 2.050 |
| R因子 | 0.192 |
| Rwork | 0.190 |
| R-free | 0.22900 |
| Structure solution method | MAD |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.026 |
| 結合角の平均二乗偏差(RMSD) [度] | 2.313 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | REFMAC (refmac_5.5.0088) |
Quality characteristics
| Overall | Inner shell | Outer shell | |
| 分解能 [Å] (低) | 20.000 | 20.000 | 2.100 |
| 分解能 [Å] (高) | 2.050 | 9.170 | 2.050 |
| Rmerge_l_obs | 0.057 | 0.021 | 0.516 |
| 独立反射数 | 41716 | 480 | 3031 |
| <I/σ(I)> | 32.29 | 79.6 | 6.2 |
| 完全性 [%] | 99.8 | 85.7 | 100 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.1 M HEPES, pH 8.0, 24% PEG3350, 0.2 M NaCl, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.1 M HEPES, pH 8.0, 24% PEG3350, 0.2 M NaCl, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
