3JRQ
Crystal structure of (+)-ABA-bound PYL1 in complex with ABI1
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 21 1 |
| 格子定数 [Å] | 49.010, 60.620, 84.960 |
| 格子定数 [度] | 90.00, 104.56, 90.00 |
精密化法
| 残基 | 20.000 - 2.100 |
| R因子 | 0.201 |
| Rwork | 0.198 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1A6Q and 3JRS |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.023 |
| 結合角の平均二乗偏差(RMSD) [度] | 2.169 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | REFMAC (refmac_5.5.0088) |
Quality characteristics
| Overall | Inner shell | Outer shell | |
| 分解能 [Å] (低) | 20.000 | 20.000 | 2.150 |
| 分解能 [Å] (高) | 2.100 | 9.390 | 2.100 |
| Rmerge_l_obs | 0.093 | 0.050 | 0.348 |
| 独立反射数 | 26472 | 290 | 1621 |
| <I/σ(I)> | 10.88 | 21.3 | 2.4 |
| 完全性 [%] | 93.3 | 82.6 | 78.1 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 21% PEG3000, 0.1 M sodium citrate pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
