3JRE
Crystal structure of Fis bound to 27 bp DNA F26 containing A-tract at center
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-02-25 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 43.638, 94.215, 155.272 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 80.580 - 3.170 |
R-factor | 0.21 |
Rwork | 0.209 |
R-free | 0.23200 |
Structure solution method | 3IV5 |
RMSD bond length | 0.014 |
RMSD bond angle | 2.061 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 90.000 | 90.000 | 3.260 |
High resolution limit [Å] | 3.150 | 6.790 | 3.150 |
Rmerge | 0.196 | 0.106 | 0.717 |
Number of reflections | 9497 | ||
<I/σ(I)> | 5.7 | ||
Completeness [%] | 83.9 | 78.2 | 86.1 |
Redundancy | 5.6 | 5.4 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 0.2 M Sodium citrate, 0.1 M TRIS-HCl pH 8.5, 36% PEG 400, vapor diffusion, hanging drop, temperature 277K |